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Please use this identifier to cite or link to this item: http://arks.princeton.edu/ark:/88435/dsp01707959895
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dc.contributor.advisorBassler, Bonnieen_US
dc.contributor.authorDonnell, Zachen_US
dc.contributor.otherMolecular Biology Departmenten_US
dc.date.accessioned2015-03-26T14:30:12Z-
dc.date.available2015-03-26T14:30:12Z-
dc.date.issued2015en_US
dc.identifier.urihttp://arks.princeton.edu/ark:/88435/dsp01707959895-
dc.description.abstractQuorum sensing (QS) is a cell-to-cell communication mechanism used by bacteria to determine their population density and to regulate genes involved in group behaviors. The human pathogen Vibrio cholerae contains two distinct QS systems that converge into a shared phosphorelay pathway. The cholera-specific pathway is composed of the autoinducer CAI-1, which is produced by the synthase CqsA and recognized by the membrane-bound histidine kinase CqsS. The universal QS pathway is composed of the autoinducer AI-2, which is produced by the autoinducer synthase LuxS and binds to the receptor LuxPQ. Here, we investigate regulation of the V. cholerae autoiducer synthases and receptors. We discovered that the LuxS/PQ system exhibits no regulation under conditions examined, whereas the CqsA/S system contains an auto-regulatory positive feedback loop. We characterized feedback in the CqsA/S by identifying the Qrr sRNAs as the source of feedback, and that they act via indirect repression of CqsA/S through an unknown intermediate. Additionally, we showed that regulation of cqsA and cqsS occurs at the post-transcriptional level. We further investigated growth phase regulation of cqsA, and discovered DctD as a potential regulator of the Qrr sRNAs.en_US
dc.language.isoenen_US
dc.publisherPrinceton, NJ : Princeton Universityen_US
dc.relation.isformatofThe Mudd Manuscript Library retains one bound copy of each dissertation. Search for these copies in the <a href=http://catalog.princeton.edu> library's main catalog </a>en_US
dc.subjectbacteriaen_US
dc.subjectcqsAen_US
dc.subjectcqsSen_US
dc.subjectquorum sensingen_US
dc.subjectsRNAen_US
dc.subjectVibrio choleraeen_US
dc.subject.classificationMolecular biologyen_US
dc.subject.classificationMicrobiologyen_US
dc.subject.classificationGeneticsen_US
dc.titleRegulation of cqsA and cqsS in Vibrio choleraeen_US
dc.typeAcademic dissertations (Ph.D.)en_US
pu.projectgrantnumber690-2143en_US
Appears in Collections:Molecular Biology

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